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The ANSI/IICRC S540 Standard describes the procedures to be followed and the precautions to be taken when performing trauma and crime scene cleanup regardless of surface, item, or location. It defines criteria and methodology used by the technician for inspecting and investigating blood and Other Potentially Infectious Material (OPIM) contamination and for establishing work plans and procedures. This Standard assumes that all scenes have been released by law enforcement or regulatory agencies.

Trauma and crime scene cleanup consists of the following components for which procedures are described in this Standard:

• Principles of Trauma and Crime Scene Cleanup;
• Safety and Health;
• Antimicrobial (Biocide) Technology;
• Administrative Procedures, Project Documentation, and Risk Management;
• Inspection and Preliminary Determination;
• Equipment and Supplies;
• Limitations, Complexities, Complications, and Conflicts;
• Structural Remediation;
• Vehicle and Other Machinery Remediation;
• Contents Remediation;
• Packaging, Transport, and Disposal of Regulated Infectious or Potentially Infectious Waste; and
• Confirmation of Cleaning.